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Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys
(Plasmid #15602)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15602 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAED4
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sup35 NM
  • Alt name
    Sup35
  • Species
    S. cerevisiae (budding yeast); Candida albicans
  • Insert Size (bp)
    830
  • Mutation
    4 G/A. Four glycines mutated to alanines. The position of the residues in the chimera are 64, 65, 74, 75. The position of the residues in CaNM are 70, 71, 80, 81.
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
  • Tags / Fusion Proteins
    • 7xHis (C terminal on insert)
    • Cysteine (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jonathan Weissman's lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Protein expression inducible by IPTG. Sc/Ca = Saccharomyces cerevisiae / Candida albicans chimera (residues ScNM 1-40, CaNM 49-141, ScNM 124-254), NM = N-terminal and Middle domains of Sup35.

Lindquist plasmid # 8635.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys was a gift from Susan Lindquist (Addgene plasmid # 15602 ; http://n2t.net/addgene:15602 ; RRID:Addgene_15602)

  • For your References section:

    Prion recognition elements govern nucleation, strain specificity and species barriers. Tessier PM, Lindquist S. Nature. 2007 May 31. 447(7144):556-61. 10.1038/nature05848 PubMed 17495929
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