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Addgene

Sup35 CaNM K227C His7
(Plasmid #15599)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAED4
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sup35 NM
  • Alt name
    Sup35
  • Species
    Candida albicans
  • Insert Size (bp)
    900
  • Mutation
    K227C. Lysine 227 mutated to cysteine.
  • Tag / Fusion Protein
    • 7xHis (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jonathan Weissman's lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Protein expression inducible by IPTG. Ca = Candida albicans, NM = N-terminal and Middle domains of Sup35.

Lindquist plasmid # 8632.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sup35 CaNM K227C His7 was a gift from Susan Lindquist (Addgene plasmid # 15599 ; http://n2t.net/addgene:15599 ; RRID:Addgene_15599)

  • For your References section:

    Prion recognition elements govern nucleation, strain specificity and species barriers. Tessier PM, Lindquist S. Nature. 2007 May 31. 447(7144):556-61. 10.1038/nature05848 PubMed 17495929
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