Sup35 ScNM A230C His7
(Plasmid #15598)

• Depositing Lab: Susan Lindquist
• Publication: Tessier et al Nature. 2007 May 31. 447(7144):556-61.

Backbone

• Vector backbone: pAED4
• Backbone size w/o insert (bp): 3200
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Sup35 NM
• Alt name: Sup35
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 780
• Mutation: A230C. Alanine 230 mutated to cysteine.
• Entrez Gene: SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
• Tag / Fusion Protein:
  • 7xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jonathan Weissman's lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Protein expression inducible by IPTG. Sc = Saccharomyces cerevisiae, NM = N-terminal and Middle domains of Sup35.

Lindquist plasmid # 8631.