CUP Puf2 YFP p316
(Plasmid
#15595)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15595 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep316 CUP
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePUF2
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SpeciesS. cerevisiae (budding yeast)
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Entrez GenePUF2 (a.k.a. YPR042C)
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Tag
/ Fusion Protein
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Figure 5 in article.
Lindquist lab number: 8030
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CUP Puf2 YFP p316 was a gift from Susan Lindquist (Addgene plasmid # 15595 ; http://n2t.net/addgene:15595 ; RRID:Addgene_15595) -
For your References section:
A network of protein interactions determines polyglutamine toxicity. Duennwald ML, Jagadish S, Giorgini F, Muchowski PJ, Lindquist S. Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11051-6. 10.1073/pnas.0604548103 PubMed 16832049