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Addgene

MET FLAG25QDProCFPp303
(Plasmid #15588)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15588 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p303 Met
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    htt 25Q Delta Pro
  • Alt name
    htt 25Q
  • Species
    H. sapiens (human)
  • Mutation
    Proline-rich region removed
  • GenBank ID
    NM_002111
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • CFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Figure 1C in article.

Lindquist lab number: 8053

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MET FLAG25QDProCFPp303 was a gift from Susan Lindquist (Addgene plasmid # 15588)

  • For your References section:

    Flanking sequences profoundly alter polyglutamine toxicity in yeast. Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S. Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50. 10.1073/pnas.0604547103 PubMed 16832050
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