GAL FLAG72QDProCFPp303
(Plasmid
#15586)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15586 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep303 Gal
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehtt 72Q Delta Pro
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Alt namehtt 72Q
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SpeciesH. sapiens (human)
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MutationProline-rich region removed
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GenBank IDNM_002111
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Entrez GeneHTT (a.k.a. HD, IT15, LOMARS)
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Tags
/ Fusion Proteins
- FLAG (N terminal on insert)
- CFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Gal1
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Figure 1C in article.
Lindquist lab number: 7732
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GAL FLAG72QDProCFPp303 was a gift from Susan Lindquist (Addgene plasmid # 15586 ; http://n2t.net/addgene:15586 ; RRID:Addgene_15586) -
For your References section:
Flanking sequences profoundly alter polyglutamine toxicity in yeast. Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S. Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50. 10.1073/pnas.0604547103 PubMed 16832050