GAL FLAG103Q+ProGFPp303
(Plasmid #15573)

• Depositing Lab: Susan Lindquist
• Publication: Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.

Backbone

• Vector backbone: p303 Gal
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: htt 103Q + Pro
• Alt name: htt 103Q
• Species: H. sapiens (human)
• Mutation: Proline-rich region present
• GenBank ID: NM_002111
• Entrez Gene: HTT (a.k.a. HD, IT15, LOMARS)
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: Gal1
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Figure 2, IV in article.

Lindquist lab number: 7698

Due to slippage when polymerases go through them, the Q stretches often get shorter over time due to mistakes while they are replicated in the cells. The shortening should not have much of an effect until the Q stretches get into the 80s.