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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15566 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSUPER
- Backbone size w/o insert (bp) 3176
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Vector typeMammalian Expression, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMRE11 shRNA
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Alt nameMRE11
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SpeciesH. sapiens (human)
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Insert Size (bp)60
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Entrez GeneMRE11 (a.k.a. ATLD, HNGS1, MRE11A, MRE11B)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sense oligo sequence used for cloning: MRE11, 5'-GATCCCCGGC ACTGAGAAAC ATGCAATTCA AGAGATTGCA TGTTTCTCAG TGCCTTTTTG GAAA-3'.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSUPER.MRE11i was a gift from Didier Trono (Addgene plasmid # 15566 ; http://n2t.net/addgene:15566 ; RRID:Addgene_15566) -
For your References section:
Ataxia-telangiectasia-mutated (ATM) protein can enhance human immunodeficiency virus type 1 replication by stimulating Rev function. Ariumi Y, Trono D. J Virol. 2006 Mar . 80(5):2445-52. 10.1128/JVI.80.5.2445-2452.2006 PubMed 16474151