pGEX-5X1 Flag PA1
(Plasmid
#15560)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15560 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-5X1
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4972
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePA1
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SpeciesH. sapiens (human)
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Insert Size (bp)1600
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GenBank IDBC003640
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Entrez GenePAGR1 (a.k.a. C16orf53, GAS, PA1)
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-5X1 Flag PA1 was a gift from Kai Ge (Addgene plasmid # 15560 ; http://n2t.net/addgene:15560 ; RRID:Addgene_15560) -
For your References section:
PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex. Cho YW, Hong T, Hong S, Guo H, Yu H, Kim D, Guszczynski T, Dressler GR, Copeland TD, Kalkum M, Ge K. J Biol Chem. 2007 Jul 13. 282(28):20395-406. 10.1074/jbc.M701574200 PubMed 17500065
Map uploaded by the depositor.