Skip to main content
Addgene

pWZLneo Flag hDPY30
(Plasmid #15555)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15555 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pWZL neo
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DPY30
  • Alt name
    DPY-30
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    400
  • GenBank ID
    NM_032574
  • Entrez Gene
    DPY30 (a.k.a. Cps25, HDPY-30, Saf19)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

FLAG-tagged hDPY-30 was cloned by PCR from HeLaS cDNA using primers 5'-C AGT AGG ATC CTG GCC ACC ATG GAC TAC AAG GAC GAC GAT GAC AAG ATG GAG CCA GAG CAG ATG CTG G-3' and 5'-TCG CTG CTC GAG TCA GTT TCG ATC TTC AAA CTG TGC-3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZLneo Flag hDPY30 was a gift from Kai Ge (Addgene plasmid # 15555 ; http://n2t.net/addgene:15555 ; RRID:Addgene_15555)

  • For your References section:

    PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex. Cho YW, Hong T, Hong S, Guo H, Yu H, Kim D, Guszczynski T, Dressler GR, Copeland TD, Kalkum M, Ge K. J Biol Chem. 2007 Jul 13. 282(28):20395-406. 10.1074/jbc.M701574200 PubMed 17500065
Related items:
Commonly requested with: