pGEX-5X1 Ash2L full
(Plasmid #15549)

• Depositing Lab: Kai Ge
• Publication: Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-406.

Backbone

• Vector backbone: pGEX-5X1
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4972
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ash2L
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• GenBank ID: BC015936
• Entrez Gene: ASH2L (a.k.a. ASH2, ASH2L1, ASH2L2, Bre2)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX 5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full-length ASH2L cDNA was amplified by reverse transcription-PCR from HeLaS cells using primers 5'-TAT AGG ATC CTG GTG ATG GCG GCG GCA GGA G-3' and 5'-CAG CGA CTC GAG TCA GGG TTC CCA TGG GGG AC-3'.