pKH3-p190
(Plasmid #15547)

• Purpose: Expression of HA-tagged p190 RhoGAP
• Depositing Lab: Ian Macara
• Publication: Tatsis et al J Biol Chem. 1998 Dec 18. 273(51):34631-8.

Backbone

• Vector backbone: pKH3
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p190 GTPase activating protein
• Alt name: p190
• Species: R. norvegicus (rat)
• Insert Size (bp): 4500
• GenBank ID: M94721
• Entrez Gene: Arhgap35 (a.k.a. Grlf1, p190RhoGAP)
• Tag / Fusion Protein:
  • Triple HA1 tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The HindIII-EcoRI fragment used to produce the plasmid was isolated from p190 cDNA clone 391 provided by R. Weinberg and J. Settleman (Massachusetts Institute of Technology).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Digesting the plasmid with BamHI+EcoRI will excise the entire full-length p190 ORF.