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Addgene

Tbr1
(Plasmid #15543)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15543 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    E61 (pBS derivative)
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tbr1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    260
  • Entrez Gene
    Tbr1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make antisense probe, cut with HindIII and transcribe with T3 RNA polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tbr1 was a gift from John Rubenstein (Addgene plasmid # 15543 ; http://n2t.net/addgene:15543 ; RRID:Addgene_15543)

  • For your References section:

    T-brain-1: a homolog of Brachyury whose expression defines molecularly distinct domains within the cerebral cortex. Bulfone A, Smiga SM, Shimamura K, Peterson A, Puelles L, Rubenstein JL. Neuron. 1995 Jul . 15(1):63-78. 10.1016/0896-6273(95)90065-9 PubMed 7619531
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