Nkx2.1
(Plasmid #15540)

• Depositing Lab: John Rubenstein
• Publication: Shimamura et al Development. 1995 Dec . 121(12):3923-33.

Backbone

• Vector backbone: E61 (pBS derivative)
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nkx2.1
• Alt name: Nkx-2.1
• Species: M. musculus (mouse)
• Insert Size (bp): 2200
• Entrez Gene: Nkx2-1 (a.k.a. Nkx2.1, T/EBP, Titf1, Ttf-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI or ClaI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To make antisense probe, cut with SalI and transcribe with T3 RNA polymerase. To make sense probe, cut with NotI and transcribe with T7 RNA polymerase.