pJK528
(Plasmid
#155388)
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PurposedCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 155388 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOSIP-KO
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Backbone manufacturerDrew Endy, Keith Shearwin (Addgene #45985)
- Total vector size (bp) 12613
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Pir1
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Growth instructionsMay require 48 hours for growth on plates.
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namedCas12a (F. novicida)
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Alt namedCpf1
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SpeciesFrancisella tularensis subsp. novicida
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Insert Size (bp)3903
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MutationD917A (nuclease-deactivating)
Gene/Insert 2
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Gene/Insert namePA4-mVenus
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Insert Size (bp)997
Gene/Insert 3
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Gene/Insert namedCas12a oscillator crRNAs
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SpeciesSynthetic
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Insert Size (bp)578
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byFeng Zhang (Addgene #69973); pLPT119 (Johan Paulsson, Addgene #85526)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK528 was a gift from Pamela Silver (Addgene plasmid # 155388 ; http://n2t.net/addgene:155388 ; RRID:Addgene_155388) -
For your References section:
Toward a translationally independent RNA-based synthetic oscillator using deactivated CRISPR-Cas. Kuo J, Yuan R, Sanchez C, Paulsson J, Silver PA. Nucleic Acids Res. 2020 Aug 20;48(14):8165-8177. doi: 10.1093/nar/gkaa557. 10.1093/nar/gkaa557 PubMed 32609820