pJK503
(Plasmid #155380)

• Purpose: dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus
• Depositing Lab: Pamela Silver
• Publication: Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):8165-8177. doi: 10.1093/nar/gkaa557.

Backbone

• Vector backbone: SC101
• Total vector size (bp): 9573
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: dCas12a (F. novicida)
• Alt name: dCpf1
• Species: Francisella tularensis subsp. novicida
• Insert Size (bp): 3903
• Mutation: D917A (nuclease-deactivating)

Gene/Insert 2

• Gene/Insert name: PA4-mVenus
• Insert Size (bp): 997

Gene/Insert 3

• Gene/Insert name: dCas12a oscillator crRNAs
• Species: Synthetic
• Insert Size (bp): 578

Resource Information

• Supplemental Documents:
  • pJK503_PAS.gb
• A portion of this plasmid was derived from a plasmid made by: Feng Zhang (Addgene #69973); pLPT119 (Johan Paulsson, Addgene #85526)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry