Dlx5
(Plasmid #15538)

• Depositing Lab: John Rubenstein
• Publication: Zerucha et al J Neurosci. 2000 Jan 15. 20(2):709-21.

Backbone

• Vector backbone: pBluescript KS
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dlx5
• Species: M. musculus (mouse)
• Insert Size (bp): 900
• GenBank ID: AAB94580.1
• Entrez Gene: Dlx5

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To make antisense probe, cut with HindIII, SalI or XhoI and transcribe with T7 RNA polymerase. To make sense probe, cut with XbaI, NotI or SacI and transcribe with T3 RNA polymerase.