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Addgene

pJK422
(Plasmid #155373)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155373 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOSIP-KO
  • Backbone manufacturer
    Drew Endy, Keith Shearwin (Addgene #45985)
  • Total vector size (bp) 12893
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    May require 48 hours for growth on plates.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    dCas9 (bacteria)
  • Alt name
    deactivated Cas9 (S. pyogenes)
  • Species
    S. pyogenes
  • Insert Size (bp)
    4107
  • Mutation
    D10A H840A (catalytically inactive)

Gene/Insert 2

  • Gene/Insert name
    PA4-mVenus
  • Insert Size (bp)
    997

Gene/Insert 3

  • Gene/Insert name
    dCas9 sgRNA oscillator v1
  • Species
    Synthetic
  • Insert Size (bp)
    619

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pdCas9-bacteria (Stanley Qi, Addgene #44249); pLPT119 (Johan Paulsson, Addgene #85526); Christopher Voigt (Addgene #62307, 62308)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK422 was a gift from Pamela Silver (Addgene plasmid # 155373 ; http://n2t.net/addgene:155373 ; RRID:Addgene_155373)

  • For your References section:

    Toward a translationally independent RNA-based synthetic oscillator using deactivated CRISPR-Cas. Kuo J, Yuan R, Sanchez C, Paulsson J, Silver PA. Nucleic Acids Res. 2020 Aug 20;48(14):8165-8177. doi: 10.1093/nar/gkaa557. 10.1093/nar/gkaa557 PubMed 32609820
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