Dlx2
(Plasmid #15537)

• Depositing Lab: John Rubenstein
• Publication: Porteus et al Neuron. 1991 Aug . 7(2):221-9.

Backbone

• Vector backbone: E61 (pBS derivative)
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dlx2
• Species: M. musculus (mouse)
• Insert Size (bp): 1000
• Mutation: Partial cDNA
• Entrez Gene: Dlx2 (a.k.a. DII A, Dlx-2, Tes-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To make antisense probe, cut with HindIII for full-length probe or EcoRI for 560 bp probe without the homeobox, and transcribe with T3 RNA polymerase. To make sense probe, cut with NotI and transcribe with T7 RNA polymerase.