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pOD_003
(Plasmid #155362)

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Item Catalog # Description Quantity Price (USD)
Plasmid 155362 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDEL
  • Backbone manufacturer
    Ilya B. Tikh and James C. Samuelson
  • Backbone size (bp) 4207
  • Modifications to backbone
    Replacement of KanR by Zeocin resistance cassette. Zeocin cassette was amplified from TT24692 from Roth lab ( http://rothlab.ucdavis.edu/cgi-bin/textQuery.cgi )
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    The plasmid can be maintained in Pir2 strain.
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

- The destination strain should be recA+ and not be able to maintain R6K plasmid
- Zeocin is used to select colonies with the integrated vector.
- The rhaBAD and lac promoters can be induced with 0.2% rhamnose and 0.5mM IPTG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOD_003 was a gift from Elisabeth Raleigh (Addgene plasmid # 155362 ; http://n2t.net/addgene:155362 ; RRID:Addgene_155362)

  • For your References section:

    Reassembling a cannon in the DNA defense arsenal: Genetics of StySA, a BREX phage exclusion system in Salmonella lab strains. Zaworski J, Dagva O, Brandt J, Baum C, Ettwiller L, Fomenkov A, Raleigh EA. PLoS Genet. 2022 Apr 4;18(4):e1009943. doi: 10.1371/journal.pgen.1009943. eCollection 2022 Apr. 10.1371/journal.pgen.1009943 PubMed 35377874
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