Dlx1
(Plasmid #15536)

• Depositing Lab: John Rubenstein
• Publication: Bulfone et al J Neurosci. 1993 Jul . 13(7):3155-72.

Backbone

• Vector backbone: pBS derivative
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dlx1
• Species: M. musculus (mouse)
• Insert Size (bp): 240
• Entrez Gene: Dlx1 (a.k.a. DII B, Dlx, Dlx-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Probe region is just 3' to the homeobox. To make antisense probe, cut with BamHI and transcribe with T7 RNA polymerase. To make sense probe, cut with EcoRV and transcribe with T3 RNA polymerase.