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Addgene

pJBJZ_006
(Plasmid #155359)

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Item Catalog # Description Quantity Price (USD)
Plasmid 155359 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pKD3
  • Backbone manufacturer
    Wanner and Datsenko
  • Backbone size (bp) 2948
  • Modifications to backbone
    Replacement of CamR by Zeocin resistance cassette between priming site 1 and common priming site c2. It was done by PCR amplification and HiFi assembly.
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Bleocin (Zeocin), 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    The plasmid can be maintained in Pir2 strain. Bring resistance to Zeomicin or bleomicin.
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Amplification of the R cassette for lambda-red recombination can be done with or without FRT.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJBJZ_006 was a gift from Elisabeth Raleigh (Addgene plasmid # 155359 ; http://n2t.net/addgene:155359 ; RRID:Addgene_155359)

  • For your References section:

    Reassembling a cannon in the DNA defense arsenal: Genetics of StySA, a BREX phage exclusion system in Salmonella lab strains. Zaworski J, Dagva O, Brandt J, Baum C, Ettwiller L, Fomenkov A, Raleigh EA. PLoS Genet. 2022 Apr 4;18(4):e1009943. doi: 10.1371/journal.pgen.1009943. eCollection 2022 Apr. 10.1371/journal.pgen.1009943 PubMed 35377874
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