Skip to main content
Addgene

pJBJZ_001
(Plasmid #155357)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 155357 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pKD3
  • Backbone manufacturer
    Wanner and Datsenko
  • Backbone size (bp) 3104
  • Modifications to backbone
    Replacement of CamR by Gentamycin resistance cassette between priming site 1 and common priming site c2. It was done by PCR amplification and HiFi assembly.
  • Vector type
    Synthetic Biology
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    The plasmid can be maintained in Pir2 strains as well.
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Amplification of the R cassette for lambda-red recombination can be done with or without FRT.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJBJZ_001 was a gift from Elisabeth Raleigh (Addgene plasmid # 155357 ; http://n2t.net/addgene:155357 ; RRID:Addgene_155357)

  • For your References section:

    Reassembling a cannon in the DNA defense arsenal: Genetics of StySA, a BREX phage exclusion system in Salmonella lab strains. Zaworski J, Dagva O, Brandt J, Baum C, Ettwiller L, Fomenkov A, Raleigh EA. PLoS Genet. 2022 Apr 4;18(4):e1009943. doi: 10.1371/journal.pgen.1009943. eCollection 2022 Apr. 10.1371/journal.pgen.1009943 PubMed 35377874
Related items:
Commonly requested with: