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Addgene

SZ_EC_02
(Plasmid #155341)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155341 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCfB6630
  • Backbone size w/o insert (bp) 2833
  • Total vector size (bp) 11624
  • Vector type
    Yeast Expression ; EasyCloneYALI insert vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    tef1 promoter - xylulose kinase - pex20 terminator
  • Alt name
    XK
  • Alt name
    YALI1_F14583g
  • Alt name
    YALI0E12463p
  • Species
    Yarrowia lipolytica
  • Insert Size (bp)
    2360
  • Promoter tef1

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACGCAACTAACATGAATGAATACG
  • 3′ sequencing primer ATCGCagagaccgggttggc
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    pyk1 promoter - xylose reductase - pex16 terminator
  • Alt name
    XR
  • Alt name
    YALI1_D09870g
  • Species
    Yarrowia lipolytica
  • Insert Size (bp)
    2387
  • Promoter pyk1

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aagcttcgagaagcccgaac
  • 3′ sequencing primer tgcgcgcttctgttgtttgc
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    gapdh promoter - xylitol dehydrogenase - lip2 terminator
  • Alt name
    XDH
  • Alt name
    YALI1_E15452g
  • Species
    Yarrowia lipolytica
  • Insert Size (bp)
    3024
  • Promoter gapdh

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ggttgaaatgaatcggccgac
  • 3′ sequencing primer ggttgaaatgaatcggccgac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are a few differences between the Addgene NGS result and depositor's sequence. These discrepancies do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SZ_EC_02 was a gift from Jens Nielsen (Addgene plasmid # 155341 ; http://n2t.net/addgene:155341 ; RRID:Addgene_155341)

  • For your References section:

    Tolerance of Yarrowia lipolytica to inhibitors commonly found in lignocellulosic hydrolysates. Konzock O, Zaghen S, Norbeck J. BMC Microbiol. 2021 Mar 8;21(1):77. doi: 10.1186/s12866-021-02126-0. 10.1186/s12866-021-02126-0 PubMed 33685391
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