pk7-GR-GFP
(Plasmid #15534)

• Depositing Lab: Ian Macara
• Publication: Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

Backbone

• Vector backbone: pk7-GFP
• Backbone size w/o insert (bp): 5460
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Glucocorticoid receptor
• Alt name: pk7-GR-GFP
• Alt name: pk-GR-GFP
• Alt name: GR-GFP
• Species: H. sapiens (human)
• Insert Size (bp): 2333
• Mutation: The glucocorticoid receptor was cloned into the XbaI site in pk7-GFP such that the stop codon of GR was deleted and the coding sequence continued in frame with that of the GFP
• GenBank ID: NM_001018077
• Entrez Gene: NR3C1 (a.k.a. GCCR, GCR, GCRST, GR, GRL)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CAG GTC CAA CTG CAC CTC GGT TC

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry