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pcDNA3.1_ spike_del19
(Plasmid #155297)

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Item Catalog # Description Quantity Price (USD)
Plasmid 155297 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCDNA3.1
  • Vector type
    Generation of SARS-CoV-2-pseudotyped lentiviral particles

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-Cov2 spike_deleted
  • Species
    SARS-Cov2
  • Mutation
    Deletion in C-term 19 aa of spike
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_ spike_del19 was a gift from Raffaele De Francesco (Addgene plasmid # 155297 ; http://n2t.net/addgene:155297 ; RRID:Addgene_155297)

  • For your References section:

    COVID-eVax, an electroporated DNA vaccine candidate encoding the SARS-CoV-2 RBD, elicits protective responses in animal models. Conforti A, Marra E, Palombo F, Roscilli G, Rava M, Fumagalli V, Muzi A, Maffei M, Luberto L, Lione L, Salvatori E, Compagnone M, Pinto E, Pavoni E, Bucci F, Vitagliano G, Stoppoloni D, Pacello ML, Cappelletti M, Ferrara FF, D'Acunto E, Chiarini V, Arriga R, Nyska A, Di Lucia P, Marotta D, Bono E, Giustini L, Sala E, Perucchini C, Paterson J, Ryan KA, Challis AR, Matusali G, Colavita F, Caselli G, Criscuolo E, Clementi N, Mancini N, Gross R, Seidel A, Wettstein L, Munch J, Donnici L, Conti M, De Francesco R, Kuka M, Ciliberto G, Castilletti C, Capobianchi MR, Ippolito G, Guidotti LG, Rovati L, Iannacone M, Aurisicchio L. Mol Ther. 2021 Sep 20. pii: S1525-0016(21)00466-4. doi: 10.1016/j.ymthe.2021.09.011. 10.1016/j.ymthe.2021.09.011 PubMed 34547465
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