pI7-ISH
(Plasmid
#15527)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-T Easy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3015
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Vector typefor riboprobe, in situ hybridization
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameI7
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Alt nameMOR103-15
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Alt nameOlfr2
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SpeciesM. musculus (mouse)
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Insert Size (bp)984
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GenBank IDAY073714
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Entrez GeneOr6a2 (a.k.a. I7, MOR103-15, Olfr2, Olfr41)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pI7-ISH was a gift from Peter Mombaerts (Addgene plasmid # 15527 ; http://n2t.net/addgene:15527 ; RRID:Addgene_15527) -
For your References section:
Local and cis Effects of the H Element on Expression of Odorant Receptor Genes in Mouse. Fuss SH, Omura M, Mombaerts P. Cell. 2007 Jul 27. 130(2):373-84. 10.1016/j.cell.2007.06.023 PubMed 17662950