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Watermelon backbone
(Plasmid #155258)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155258 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Watermelon
  • Backbone size (bp) 9826
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the A76V mutation in mCherry does not affect overall plasmid function. Please visit https://www.biorxiv.org/content/10.1101/2020.06.05.136358v1 for bioRxiv preprint.

Please see the Nature publication section "Cloning of the Watermelon library" to see how the backbone sequence compares to the final plasmids used in the Watermelon Library (#155257).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Watermelon backbone was a gift from Joan Brugge & Aviv Regev (Addgene plasmid # 155258 ; http://n2t.net/addgene:155258 ; RRID:Addgene_155258)

  • For your References section:

    Cycling cancer persister cells arise from lineages with distinct programs. Oren Y, Tsabar M, Cuoco MS, Amir-Zilberstein L, Cabanos HF, Hutter JC, Hu B, Thakore PI, Tabaka M, Fulco CP, Colgan W, Cuevas BM, Hurvitz SA, Slamon DJ, Deik A, Pierce KA, Clish C, Hata AN, Zaganjor E, Lahav G, Politi K, Brugge JS, Regev A. Nature. 2021 Aug 11. pii: 10.1038/s41586-021-03796-6. doi: 10.1038/s41586-021-03796-6. 10.1038/s41586-021-03796-6 PubMed 34381210
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