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Addgene

pcDNA-rUSP19ER-siRNAresistant
(Plasmid #155251)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155251 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1/Myc-His A
  • Backbone size w/o insert (bp) 5493
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    USP19 (ER isoform) siRNA resistant
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    4074
  • Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG to CAA
  • Entrez Gene
    Usp19
  • Promoter CMV
  • Tag / Fusion Protein
    • 6XHis-3XFlag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site PmeI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHreverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-rUSP19ER-siRNAresistant was a gift from Simon Wing (Addgene plasmid # 155251 ; http://n2t.net/addgene:155251 ; RRID:Addgene_155251)
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