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Addgene

L1-YA
(Plasmid #15525)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15525 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAS2
  • Backbone size w/o insert (bp) 8400
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    L1-cytoplasmic domain-Y1176A
  • Alt name
    L1-CAM
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    350
  • Mutation
    Tyrosine 1176 changed to Alanine
  • Entrez Gene
    L1CAM (a.k.a. CAML1, CD171, HSAS, HSAS1, HYCX, MASA, MIC5, N-CAM-L1, N-CAML1, NCAM-L1, S10, SPG1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L1-YA was a gift from Vance Lemmon (Addgene plasmid # 15525 ; http://n2t.net/addgene:15525 ; RRID:Addgene_15525)

  • For your References section:

    RanBPM is an L1-interacting protein that regulates L1-mediated mitogen-activated protein kinase activation. Cheng L, Lemmon S, Lemmon V. J Neurochem. 2005 Aug . 94(4):1102-10. 10.1111/j.1471-4159.2005.03254.x PubMed 16000162
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