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Addgene

Ad-HisFlag-USP19ER
(Plasmid #155244)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155244 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAdEasy/pShuttle-IRES-hrGFP-1
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 38900
  • Vector type
    Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    USP19 (ER isoform)
  • Alt name
    GFP
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    4074
  • Mutation
    H118R- please see dep. comments
  • Entrez Gene
    Usp19
  • Promoter CMV
  • Tag / Fusion Protein
    • 6XHis-3XFlag (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms H118R mutation does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ad-HisFlag-USP19ER was a gift from Simon Wing (Addgene plasmid # 155244 ; http://n2t.net/addgene:155244 ; RRID:Addgene_155244)

  • For your References section:

    USP19 deubiquitinating enzyme inhibits muscle cell differentiation by suppressing unfolded-protein response signaling. Wiles B, Miao M, Coyne E, Larose L, Cybulsky AV, Wing SS. Mol Biol Cell. 2015 Mar 1;26(5):913-23. doi: 10.1091/mbc.E14-06-1129. Epub 2015 Jan 7. 10.1091/mbc.E14-06-1129 PubMed 25568336
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