Skip to main content
Addgene

pcDNA-HisFlag-rUSP19CYT
(Plasmid #155238)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155238 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA 3.1/Myc-His A
  • Backbone size w/o insert (bp) 5493
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    USP19 (Cytoplasmic isoform)
  • Species
    M. musculus (mouse), R. norvegicus (rat)
  • Insert Size (bp)
    3969
  • Entrez Gene
    Usp19
  • Promoter CMV
  • Tag / Fusion Protein
    • 6XHis-3XFlag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site PmeI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHreverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-HisFlag-rUSP19CYT was a gift from Simon Wing (Addgene plasmid # 155238 ; http://n2t.net/addgene:155238 ; RRID:Addgene_155238)
Related items: