C1-CFP-Lyn11
(Plasmid
#155234)
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Purposemembane marker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 155234 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLyn11 membrane targeted CFP. Membrane control
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- CFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C1-CFP-Lyn11 was a gift from Tobias Meyer (Addgene plasmid # 155234 ; http://n2t.net/addgene:155234 ; RRID:Addgene_155234) -
For your References section:
Membrane-proximal F-actin restricts local membrane protrusions and directs cell migration. Bisaria A, Hayer A, Garbett D, Cohen D, Meyer T. Science. 2020 Jun 12;368(6496):1205-1210. doi: 10.1126/science.aay7794. 10.1126/science.aay7794 PubMed 32527825