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Addgene

C1-iRFP-iLid-CaaX
(Plasmid #155229)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155229 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iRFP C-term tagged with the ilID motif and the CaaX sequence derived from Kras4b. Membrane control
  • Species
    Synthetic
  • Tag / Fusion Protein
    • iRFP-iLid (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C1-iRFP-iLid-CaaX was a gift from Tobias Meyer (Addgene plasmid # 155229 ; http://n2t.net/addgene:155229 ; RRID:Addgene_155229)

  • For your References section:

    Membrane-proximal F-actin restricts local membrane protrusions and directs cell migration. Bisaria A, Hayer A, Garbett D, Cohen D, Meyer T. Science. 2020 Jun 12;368(6496):1205-1210. doi: 10.1126/science.aay7794. 10.1126/science.aay7794 PubMed 32527825
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