Skip to main content
Addgene

C1-mCitrine-FKBP-EZRabd(t567D)
(Plasmid #155227)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155227 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
  • Species
    H. sapiens (human)
  • Mutation
    Changed Thr 567 to Asp in original Ezrin
  • Tag / Fusion Protein
    • mCitrine (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C1-mCitrine-FKBP-EZRabd(t567D) was a gift from Tobias Meyer (Addgene plasmid # 155227 ; http://n2t.net/addgene:155227 ; RRID:Addgene_155227)

  • For your References section:

    Membrane-proximal F-actin restricts local membrane protrusions and directs cell migration. Bisaria A, Hayer A, Garbett D, Cohen D, Meyer T. Science. 2020 Jun 12;368(6496):1205-1210. doi: 10.1126/science.aay7794. 10.1126/science.aay7794 PubMed 32527825
Related items:
Commonly requested with: