C1-Lyn-mCitrine-EEVEE-LifeAct
(Plasmid
#155226)
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PurposeAlternate MPAct using Lyn/LifeAct instead of CaaX/F-tractin
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 155226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLyn11 fused to mCitrine C-term tagged with a codon optimized EEVEE and LifeAct
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SpeciesSynthetic
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Tag
/ Fusion Protein
- mCitrine (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C1-Lyn-mCitrine-EEVEE-LifeAct was a gift from Tobias Meyer (Addgene plasmid # 155226 ; http://n2t.net/addgene:155226 ; RRID:Addgene_155226) -
For your References section:
Membrane-proximal F-actin restricts local membrane protrusions and directs cell migration. Bisaria A, Hayer A, Garbett D, Cohen D, Meyer T. Science. 2020 Jun 12;368(6496):1205-1210. doi: 10.1126/science.aay7794. 10.1126/science.aay7794 PubMed 32527825