pCDNA3.1-Muc2D1D2D3cysD1
(Plasmid
#155214)
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PurposeExpresses human MUC2 residues 21-1397
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 155214 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha ; XL1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMuc2 D1D2D3cysD1
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SpeciesH. sapiens (human)
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Entrez GeneMUC2 (a.k.a. MLP, MUC-2, SMUC)
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Tag
/ Fusion Protein
- 6xHistadine (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.1-Muc2D1D2D3cysD1 was a gift from Deborah Fass (Addgene plasmid # 155214 ; http://n2t.net/addgene:155214 ; RRID:Addgene_155214) -
For your References section:
Assembly Mechanism of Mucin and von Willebrand Factor Polymers. Javitt G, Khmelnitsky L, Albert L, Bigman LS, Elad N, Morgenstern D, Ilani T, Levy Y, Diskin R, Fass D. Cell. 2020 Oct 29;183(3):717-729.e16. doi: 10.1016/j.cell.2020.09.021. Epub 2020 Oct 7. 10.1016/j.cell.2020.09.021 PubMed 33031746