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Addgene

pET_4E2H.210
(Plasmid #155212)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155212 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET11b
  • Total vector size (bp) 5874
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    4E2H.210
  • Species
    Synthetic
  • Insert Size (bp)
    291
  • Tag / Fusion Protein
    • 6x His Tag (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET_4E2H.210 was a gift from Bruno Correia (Addgene plasmid # 155212 ; http://n2t.net/addgene:155212 ; RRID:Addgene_155212)

  • For your References section:

    Bottom-up de novo design of functional proteins with complex structural features. Yang C, Sesterhenn F, Bonet J, van Aalen EA, Scheller L, Abriata LA, Cramer JT, Wen X, Rosset S, Georgeon S, Jardetzky T, Krey T, Fussenegger M, Merkx M, Correia BE. Nat Chem Biol. 2021 Apr;17(4):492-500. doi: 10.1038/s41589-020-00699-x. Epub 2021 Jan 4. 10.1038/s41589-020-00699-x PubMed 33398169
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