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Addgene

SQ171/pCSacB/ptRNA100
(Bacterial strain #155206)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 155206 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    N/A

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    SQ171/pCSacB/ptRNA100
  • Copy number
    Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Strain is described in PMID: 32518240. Genotype: ilvG rfb-50 rph-1 ΔrrnGADEHBC DEL(recA)/ ptRNA100, pA15, SpcR, alaT, aspT, ileT, trpT, gltT / pCSacB. pCSacB description (PMID: 26438293 and PMID:14617656). Strain is also resistant to spectinomycin,

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SQ171/pCSacB/ptRNA100 was a gift from Alexander Mankin (Addgene plasmid # 155206)

  • For your References section:

    Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly. Huang S, Aleksashin NA, Loveland AB, Klepacki D, Reier K, Kefi A, Szal T, Remme J, Jaeger L, Vazquez-Laslop N, Korostelev AA, Mankin AS. Nat Commun. 2020 Jun 9;11(1):2900. doi: 10.1038/s41467-020-16694-8. 10.1038/s41467-020-16694-8 PubMed 32518240
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