pDUAL_lacZ
(Plasmid #155169)

• Purpose: BaSP and CuCBP co-expression: lacZ promoter controls BaSP expression
• Depositing Lab: Bernd Nidetzky
• Publication: Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi: 10.1002/biot.202000063.

Backbone

• Vector backbone: self assembled
• Backbone size w/o insert (bp): 3142
• Total vector size (bp): 7185
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use E. coli BL21(DE3) for expression and grow at room temperature.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Sucrose Phosphorylase from Bifidobacterium adolescentis
• Alt name: BaSP
• Species: Bifidobacterium adolescentis
• Insert Size (bp): 1515
• GenBank ID: AF543301.1
• Entrez Gene: BAD_RS00415 (a.k.a. BAD_RS00415, BAD_0078)
• Tag / Fusion Protein:
  • Strep - Tag II (N terminal on backbone)

Gene/Insert 2

• Gene/Insert name: Cellobiose phosphorylase from Cellulomonas uda
• Alt name: CuCBP
• Species: Cellulomonas uda
• Insert Size (bp): 2466
• GenBank ID: AAQ20920.1
• Tag / Fusion Protein:
  • His Tag (N terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry