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Addgene

pBEL2081
(Plasmid #155161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155161 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 5531
  • Total vector size (bp) 6702

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MlaF-(6xHis-TEV-MlaB)
  • Species
    E. coli
  • Insert Size (bp)
    1171
  • Mutation
    MlaF(Y261A_L265A_L266A)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEL2081 was a gift from Gira Bhabha & Damian Ekiert (Addgene plasmid # 155161 ; http://n2t.net/addgene:155161 ; RRID:Addgene_155161)

  • For your References section:

    Structure of MlaFB uncovers novel mechanisms of ABC transporter regulation. Kolich LR, Chang YT, Coudray N, Giacometti SI, MacRae MR, Isom GL, Teran EM, Bhabha G, Ekiert DC. Elife. 2020 Jun 30;9. pii: 60030. doi: 10.7554/eLife.60030. 10.7554/eLife.60030 PubMed 32602838
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