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Addgene

H-ACNF-H
(Plasmid #15515)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15515 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSIIKS-
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    H element
  • Alt name
    H region
  • Alt name
    H enhancer
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    13814
  • Mutation
    Targeting vector to delete the 2,119 nt sequence, ScaI restriction fragment, known as the H element.
  • GenBank ID
    DQ086467

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII, NotI (not destroyed)
  • 3′ cloning site SalI, XhoI, KpnI (not destroyed)
  • 5′ sequencing primer M13 Forward
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Subclone H2.8.E.

The mouse strain that was derived from this plasmid is available from The Jackson Laboratory: http://jaxmice.jax.org/strain/006790.html

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H-ACNF-H was a gift from Peter Mombaerts (Addgene plasmid # 15515 ; http://n2t.net/addgene:15515 ; RRID:Addgene_15515)

  • For your References section:

    Local and cis Effects of the H Element on Expression of Odorant Receptor Genes in Mouse. Fuss SH, Omura M, Mombaerts P. Cell. 2007 Jul 27. 130(2):373-84. 10.1016/j.cell.2007.06.023 PubMed 17662950
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