pBEL1307
(Plasmid
#155145)
-
PurposeExpresses MlaFB, His tag on MlaB N-term
-
Depositing Labs
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 155145 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET21a
-
Backbone manufacturerThermo Fisher
- Backbone size w/o insert (bp) 5531
- Total vector size (bp) 6678
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMlaF-(6xHis-TEV-MlaB)
-
SpeciesE. coli
-
Insert Size (bp)5531
- Promoter T7 promoter
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBEL1307 was a gift from Gira Bhabha & Damian Ekiert (Addgene plasmid # 155145 ; http://n2t.net/addgene:155145 ; RRID:Addgene_155145) -
For your References section:
Structure of MlaFB uncovers novel mechanisms of ABC transporter regulation. Kolich LR, Chang YT, Coudray N, Giacometti SI, MacRae MR, Isom GL, Teran EM, Bhabha G, Ekiert DC. Elife. 2020 Jun 30;9. pii: 60030. doi: 10.7554/eLife.60030. 10.7554/eLife.60030 PubMed 32602838
