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pGEX-HA-GlpG(D243G/L244G/F245G/M247G/S248G/M249G/A250G)
(Plasmid #155136)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155136 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX 6P-1
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 5730
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GlpG
  • Species
    Escherichia coli
  • Insert Size (bp)
    828
  • Mutation
    D243G/L244G/F245G/M247G/S248G/M249G/A250G
  • GenBank ID
    NC_000913.3
  • Entrez Gene
    glpG (a.k.a. b3424, ECK3410)
  • Promoter tac
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-HA-GlpG(D243G/L244G/F245G/M247G/S248G/M249G/A250G) was a gift from Sinisa Urban (Addgene plasmid # 155136 ; http://n2t.net/addgene:155136 ; RRID:Addgene_155136)

  • For your References section:

    Ten catalytic snapshots of rhomboid intramembrane proteolysis from gate opening to peptide release. Cho S, Baker RP, Ji M, Urban S. Nat Struct Mol Biol. 2019 Oct;26(10):910-918. doi: 10.1038/s41594-019-0296-9. Epub 2019 Sep 30. 10.1038/s41594-019-0296-9 PubMed 31570873
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