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pET15-His-∆NGlpG(C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G)
(Plasmid #155134)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155134 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 6340
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GlpG
  • Species
    Escherichia coli
  • Insert Size (bp)
    636
  • Mutation
    C104A/D243G/L244G/F245G/M247C/S248G/M249G/A250G, insert codes for GlpG residues 87-276
  • GenBank ID
    NC_000913.3
  • Entrez Gene
    glpG (a.k.a. b3424, ECK3410)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter primer
  • 3′ sequencing primer T7 terminator primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15-His-∆NGlpG(C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G) was a gift from Sinisa Urban (Addgene plasmid # 155134 ; http://n2t.net/addgene:155134 ; RRID:Addgene_155134)

  • For your References section:

    Ten catalytic snapshots of rhomboid intramembrane proteolysis from gate opening to peptide release. Cho S, Baker RP, Ji M, Urban S. Nat Struct Mol Biol. 2019 Oct;26(10):910-918. doi: 10.1038/s41594-019-0296-9. Epub 2019 Sep 30. 10.1038/s41594-019-0296-9 PubMed 31570873
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