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Addgene

OMP-SpH-ACNF TV
(Plasmid #15513)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15513 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM5
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    olfactory marker protein
  • Alt name
    OMP
  • Alt name
    Omp
  • Species
    M. musculus (mouse)
  • Mutation
    OMP coding region and 3' end were replaced by synaptopHluorin in this targeting vector.
  • GenBank ID
    BC132327
  • Entrez Gene
    Omp

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ? (unknown if destroyed)
  • 3′ cloning site ? (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synaptopHluorin was obtained from Dr. James Rothman

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Targeting vector can be linearized with XhoI.

The mouse strain that was derived from this plasmid is available from The Jackson Laboratory: http://jaxmice.jax.org/strain/004946.html

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OMP-SpH-ACNF TV was a gift from Peter Mombaerts (Addgene plasmid # 15513 ; http://n2t.net/addgene:15513 ; RRID:Addgene_15513)

  • For your References section:

    In vivo imaging of neuronal activity by targeted expression of a genetically encoded probe in the mouse. Bozza T, McGann JP, Mombaerts P, Wachowiak M. Neuron. 2004 Apr 8. 42(1):9-21. 10.1016/S0896-6273(04)00144-8 PubMed 15066261
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