Skip to main content
Addgene

pSR prdm3 (PRDM16 shRNA)
(Plasmid #15505)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15505 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSuper-Retro Neo + GFP
  • Backbone manufacturer
    oligoengine
  • Backbone size w/o insert (bp) 7300
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Neomycin (select with G418) ; GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRDM16 shRNA
  • Alt name
    PRDM16
  • Alt name
    Mel1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    50
  • Entrez Gene
    Prdm16 (a.k.a. 5730557K01Rik, csp1, mel1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer MSCV rev
  • 3′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The siRNA sequence for use in PRDM16 depletion experiments was 5'-GAAGAGCGUGAGUACAAAU-3'. The corresponding double-stranded DNA sequence was ligated into pSUPER-Retro (GFP-Neo) (OligoEngine) for retroviral shRNA expression. This siRNA sequence was able to specifically knock down PRDM16 protein and mRNA expression by over 75%.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSR prdm3 (PRDM16 shRNA) was a gift from Bruce Spiegelman (Addgene plasmid # 15505 ; http://n2t.net/addgene:15505 ; RRID:Addgene_15505)

  • For your References section:

    Transcriptional Control of Brown Fat Determination by PRDM16. Seale P, Kajimura S, Yang W, Chin S, Rohas LM, Uldry M, Tavernier G, Langin D, Spiegelman BM. Cell Metab. 2007 Jul . 6(1):38-54. 10.1016/j.cmet.2007.06.001 PubMed 17618855
Related items:
Commonly requested with: