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Addgene

MSCV PRDM16
(Plasmid #15504)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15504 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV puro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6300
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRDM16 (isoform 4)
  • Alt name
    Mel1 (isoform 4)
  • Alt name
    PR domain containing 16 (isoform 4)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3600
  • Entrez Gene
    Prdm16 (a.k.a. 5730557K01Rik, csp1, mel1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer MSCV
  • 3′ sequencing primer MSCV rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert from CMV-Sport6-PRDM16 from Open Biosystems
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV PRDM16 was a gift from Bruce Spiegelman (Addgene plasmid # 15504 ; http://n2t.net/addgene:15504 ; RRID:Addgene_15504)

  • For your References section:

    Transcriptional Control of Brown Fat Determination by PRDM16. Seale P, Kajimura S, Yang W, Chin S, Rohas LM, Uldry M, Tavernier G, Langin D, Spiegelman BM. Cell Metab. 2007 Jul . 6(1):38-54. 10.1016/j.cmet.2007.06.001 PubMed 17618855
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