pVenus-N-SLC35B2
(Plasmid #155038)

• Purpose: Transient expression of SLC35B2-Venus
• Depositing Lab: Yihong Ye
• Publication: Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;117(20):10865-10875. doi: 10.1073/pnas.1918617117. Epub 2020 May 4.

Backbone

• Vector backbone: pEGFP-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SLC35B2
• Alt name: PAPST1
• Species: H. sapiens (human)
• Insert Size (bp): 1299
• Mutation: See depositor comments below
• GenBank ID: NM_178148
• Entrez Gene: SLC35B2 (a.k.a. HLD26, PAPST1, SLL, UGTrel4)
• Promoter: CMV
• Tag / Fusion Protein:
  • Venus (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a F362S mutation in SLC35B2 compared to the NCBI reference sequence NP_835361.1. It is not known how this mutation affects the protein's function, however this plasmid was functional in experiments to study the localization of this protein.