pSMALB-ATF4.5
(Plasmid #155032)

• Purpose: Fluorescent reporter for ATF4 translation
• Depositing Labs: John Dick, Peter van Galen
• Publication: van Galen et al Nature. 2014 Jun 12;510(7504):268-72. doi: 10.1038/nature13228. Epub 2014 Apr 28.

Backbone

• Vector backbone: pSMALB
• Backbone manufacturer: Peter van Galen
• Total vector size (bp): 9456
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: ATF4 5' UTR
• Species: M. musculus (mouse)
• Insert Size (bp): 277
• Entrez Gene: Atf4 (a.k.a. Atf-4, C/ATF, CREB-2, CREB2, TAXREB67)

Gene/Insert 2

• Gene/Insert name: ATF4-eGFP fusion ORF
• Insert Size (bp): 858

Gene/Insert 3

• Gene/Insert name: TagBFP
• Insert Size (bp): 702

Resource Information

• Supplemental Documents:
  • pSMALB-ATF4.5.gb
• A portion of this plasmid was derived from a plasmid made by: 5' UTR and ATF4-GFP fusion was clones from Addgene Plasmid #21852, "ATF4 5: 5’ATF4.GFP".
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also used in van Galen et al., 2018, Integrated Stress Response Activity Marks Stem Cells in Normal Hematopoiesis and Leukemia, Cell Reports 25, 1109–1117 (https://doi.org/10.1016/j.celrep.2018.10.021)

This plasmid should be used with pSMALB-ATF4.12 (negative control, https://www.addgene.org/155033/) and pSMALB-ATF4.14 (positive control), https://www.addgene.org/155034/.